Contact an Account ManagerPrecolumn switching in HPLC quantitation of small amounts of microbial metabolites as an alternative method to off-line extraction
Summary
Type: HPLC
Application #: 106880
Catalog#: 720002.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x125mm
Detection: UV, 230nm, att. 80mV
Conditions: eluent: linear gradient from 16% to 28% acetonitrile in water in 12min, cleaning step of 100% acetonitrile for 1min, recondition phase under initial conditions 4min
flow rate 2ml/min
Authors: Fiedler H-P
Source: Chromatographia 19 (1984) 246 - 250
Details
Legend: Fig. 6. Reproducibility of precolumn switching in the HPLC analysis of 1718-121
and 1718-122, 50µl culture filtrate injected, a) 1st, b) 120th injection.